A Combined Light Sheet Fluorescence and Differential Interference Contrast Microscope


Light Sheet Fluorescence Microscopy (LSFM) enables imaging of specimens such as live animal embryos, multicellular aggregates, and complex biomaterials with high spatial resolutions, large fields of view, and low levels of photodamage and phototoxicity. However, LSFM does not provide information about the non-fluorescent neighborhood of these cells. In Differential Interference Contrast (DIC) microscopy, the manipulation of light passing through a specimen is used to map gradients of optical path length, providing images that do not select particular cell types, but reveal the general structure of regions of the sample.

The microscope developed by the UO inventors combines LSFM and DIC integrated into one microscope. This microscope is highly useful for biological imaging due to its merger of highly specific three-dimensional fluorescence imaging and context-providing non-fluorescence imaging.

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Jim Deane
University of Oregon
Raghuveer Parthasarathy
Ryan Baker